ILTAB/ORSTOM Division of Plant Biology-MRC7, 10666 North Torrey Pines Road, 92037, La Jolla, CA, USA.
Plant Cell Rep. 1994 Aug;13(11):647-51. doi: 10.1007/BF00232938.
Regeneration of indica rice varieties remains a limiting factor for researchers undertaking rice Iransformation experiments. As reported for japonica rice and other crops, partial desiccation of indica rice calli dramatically promotes organogenesis and leads to high regeneration ability. We are now able to obtain 66.5%, 61.1% and 73.7% of calli that regenerate plants for the indica varieties TN1, IR72 and IR64 whereas in non desiccated controls only 30.0%, 15.5% and 18.7% of calli regenerated, respectively. Plants obtained were phenotypically normal and 50% were highly fertile. Partial desiccation is a reliable and simple method for improving indica rice regeneration. It also shortens the time of in vitro culture.
再生的籼稻品种仍然是一个限制因素的研究人员进行水稻转化实验。如报告的粳稻和其他作物，部分干燥的籼稻愈伤组织显著促进器官发生，并导致高再生能力。我们现在能够获得 66.5%，61.1%和 73.7%的愈伤组织再生植物的籼稻品种 TN1，IR72 和 IR64 而在非干燥对照中只有 30.0%，15.5%和 18.7%的愈伤组织再生，分别。所获得的植株表型正常，50%的高度肥沃。部分干燥是一种可靠和简单的方法，提高籼稻再生。它还缩短了体外培养的时间。
