通过器官发生从番木瓜叶柄外植体高效再生植株。

High efficiency plant regeneration from petiole explants of Carica papaya L. through organogenesis.

机构信息

Department of Botany, Rajshahi University, 6205, Rajshahi, Bangladesh.

出版信息

Plant Cell Rep. 1993 Dec;13(2):99-102. doi: 10.1007/BF00235299.

PMID:24196296

Abstract

Callus cultures were obrained from petiole explants of Carica papaya on MS medium containing 0.5-10.5 μM α-naphthaleneacetic acid (NAA) in combination with 0.5-5 μM benzyladenine (BA). Hard-green calli were transferred to MS medium containing 100 mgl(-1) casein hydrolysate (CH) with specific BA-NAA formulation, where they developed adventitious buds within 2 weeks of culture. Maximum number of adventitious buds were obtained in 2 μM BA and 0.1 μM NAA. Shoot regeneration occurred from these adventitious buds by the end of the 4th week. Regenerated shoots were elongated in hormone-free medium and rooted in half-strength MS fortified with 3 UM NAA and 0.5 μM gibberellic acid (GA3). The regenerants were transferred to soil after acclimatization.

摘要

愈伤组织培养从木瓜的叶柄外植体在含有 0.5-10.5 μM 萘乙酸（NAA）的 MS 培养基上获得，与 0.5-5 μM 苄基腺嘌呤（BA）结合。硬绿愈伤组织转移到 MS 培养基中，含 100 mgl(-1) 酪蛋白水解物（CH），并具有特定的 BA-NAA 配方，在培养的 2 周内，它们发育出不定芽。在 2 μM BA 和 0.1 μM NAA 中获得了最大数量的不定芽。不定芽在第 4 周末发生了芽再生。再生芽在无激素培养基中伸长，并在添加 3 UM NAA 和 0.5 μM 赤霉素（GA3）的半强度 MS 中生根。再生体在适应环境后被转移到土壤中。