Sogetal Inc., 3876 Bay Center Place, 94587, Hayward, California, USA.
Plant Cell Rep. 1993 Jan;12(3):149-53. doi: 10.1007/BF00239096.
Wheat microspores were isolated, without prior anther culture, from a range of genotypes and cultured to regenerate self-fertile plants. Microspores were isolated using a microblender and competent microspores were enriched by gradient centrifugation. The use of maltose as the sole carbohydrate in the culture medium and co-culture of microspores with wheat or barley ovaries were critical for development of microspore-derived embryos. Results were also improved when spikes were pretreated by emersion of the basal ends of detached heads in water at 25°C for 2d. This procedure leads to highly reproducible production of plants.
从小麦花中直接分离小孢子，无需预先进行花药培养，然后培养这些小孢子，使其再生出可自交结实的植株。采用微型匀浆器从小麦花中分离小孢子，并用梯度离心法从小孢子中富集有活力的小孢子。在培养基中仅使用麦芽糖作为唯一的碳水化合物，并与小麦或大麦的子房共培养，这对于小孢子衍生胚胎的发育至关重要。如果在 25°C 下将去头麦穗的基部浸泡在水中 2d 来预处理麦穗，也可以提高实验结果。该程序可实现高度可重复的植物生产。
