Hillar M, Santerelli I, Stolzmann Z, Wafeeg W, Allen S, Chan J Y, Patt L M, Houck J C, Wyborny L E
Physiol Chem Phys Med NMR. 1985;17(3):325-43.
DNA and nuclear RNA fractions contain small peptides (mol. wt. 600 - 1500) attached noncovalently. A large scale isolation procedure was developed for the extraction of such peptides directly from the lysed nuclei. Further purification and fractionation was performed with the chromatography on Sephadex, silica gel and h.p.l.c. C18 reverse phase columns. H.p.l.c. fractionation yielded eleven peaks. The peptides are rich in serine, glycine, alanine and acidic amino acids. They do not contain sulphur-containing amino acids. Only occasionally tyrosine, phenylalanine, histidine, arginine, and very moderate amount of lysine are found. These peptides are active in inhibiting gene expression in cell-free systems and incorporation of labeled thymidine in L 1210 murine leukemic cell culture. Thorough and exhaustive analysis demonstrated that the isolated peptides are not degradative products of histone or nonhistone chromosomal proteins.
DNA和核RNA组分含有通过非共价连接的小肽（分子量600 - 1500）。开发了一种大规模分离程序，用于直接从裂解的细胞核中提取此类肽。通过在葡聚糖凝胶、硅胶和高效液相色谱C18反相柱上进行色谱分析，进一步进行纯化和分级分离。高效液相色谱分级分离产生了11个峰。这些肽富含丝氨酸、甘氨酸、丙氨酸和酸性氨基酸。它们不含含硫氨基酸。仅偶尔发现酪氨酸、苯丙氨酸、组氨酸、精氨酸以及极少量的赖氨酸。这些肽在无细胞系统中抑制基因表达以及在L 1210小鼠白血病细胞培养中抑制标记胸苷的掺入方面具有活性。全面而详尽的分析表明，分离出的肽不是组蛋白或非组蛋白染色体蛋白的降解产物。
