Metzner Martin, Erhard Marcel, Sammra Osama, Nagib Samy, Hijazin Muaz, Alber Jörg, Lämmler Christoph, Abdulmawjood Amir, Prenger-Berninghoff Ellen, Zschöck Michael, Hassan Abdulwahed Ahmed
RIPAC-LABOR GmbH, Potsdam-Golm, Germany.
Berl Munch Tierarztl Wochenschr. 2013 Sep-Oct;126(9-10):423-6.
In the present study four Trueperella (T.) abortisuis strains isolated from an umbilical swab, two anal swabs and from a placenta after abortion of four pigs, respectively, could successfully be identified phenotypically, by MALDI-TOF MS analysis and genotypically by amplification and sequencing of 16S rRNA gene sequence and gene sodA encoding superoxide dismutase A as additional molecular target. All four T. abortisuis were isolated together with various other bacterial species indicating that the pathogenic importance of this novel species remains unclear. However, according to the literature and to the results of the present study T. abortisuis could be recovered from samples of animals in Japan and in different microbiological laboratories in Germany emphasizing its increasing importance.
在本研究中，分别从4头猪的脐带拭子、两份肛门拭子以及流产后的胎盘分离出的4株猪流产布鲁氏菌（Trueperella abortisuis）菌株，通过基质辅助激光解吸电离飞行时间质谱（MALDI-TOF MS）分析能够成功地进行表型鉴定，通过16S rRNA基因序列扩增和测序以及编码超氧化物歧化酶A的sodA基因作为额外的分子靶点进行基因型鉴定。所有4株猪流产布鲁氏菌均与其他多种细菌一同分离出来，这表明该新物种的致病重要性仍不清楚。然而，根据文献和本研究结果，在日本的动物样本以及德国不同的微生物实验室中均能分离出猪流产布鲁氏菌，这凸显了其日益增加的重要性。
