Allouh Mohammed Z, Aldirawi Mohammed H
Department of Anatomy, Faculty of Medicine, Jordan University of Science and Technology, Irbid, Jordan.
Pak J Biol Sci. 2012 Mar 1;15(5):215-23. doi: 10.3923/pjbs.2012.215.223.
Sustanon is one of the most commonly used anabolic androgenic drugs to increase skeletal muscle mass and strength. This drug is a blend of four esterized testosterone derivatives: Testosterone propionate, testosterone phenylpropionate, testosterone isocaproate and testosterone decanoate. Little is known about the effects of this drug on skeletal muscle at the cellular level. This study aimed to investigate the influence of Sustanon on the morphology of skeletal muscle fibers and the distribution of myogenic stem cells known as Satellite Cells (SCs) during postnatal growth. We hypothesized that Sustanon-induced skeletal muscle hypertrophy is associated with an increase in the number of SCs. Robust immunocytochemical techniques and morphometric analyses were used to calculate the numbers of SCs and myonuclei within the pectoralis muscle of chickens. Also, DNA concentration and Pax7 protein levels were measured to confirm immunocytochemical findings. Sustanon significantly increased pectoralis mass and fiber size. All SC indices and the number of myonuclei increased significantly by Sustanon administration. In addition, greater DNA concentration and Pax7 protein expression were found in Sustanon-treated birds. This study indicates that Sustanon can induce avian skeletal muscle hypertrophy and that this is correlated with increased numbers of SCs and myonuclei.
长效睾酮是最常用于增加骨骼肌质量和力量的合成代谢雄激素药物之一。这种药物是四种酯化睾酮衍生物的混合物：丙酸睾酮、苯丙酸睾酮、异己酸睾酮和癸酸睾酮。关于这种药物在细胞水平对骨骼肌的影响知之甚少。本研究旨在调查长效睾酮对出生后生长期间骨骼肌纤维形态和被称为卫星细胞（SCs）的成肌干细胞分布的影响。我们假设长效睾酮诱导的骨骼肌肥大与卫星细胞数量增加有关。使用强大的免疫细胞化学技术和形态计量分析来计算鸡胸肌内卫星细胞和肌细胞核的数量。此外，测量DNA浓度和Pax7蛋白水平以确认免疫细胞化学结果。长效睾酮显著增加了胸肌质量和纤维大小。通过给予长效睾酮，所有卫星细胞指标和肌细胞核数量均显著增加。此外，在接受长效睾酮治疗的鸡中发现了更高的DNA浓度和Pax7蛋白表达。本研究表明，长效睾酮可诱导禽类骨骼肌肥大，且这与卫星细胞和肌细胞核数量增加相关。
