Chen Guang-Liang, Zhu Li-Ran, Na Sha, Li Li
Integrative Medicine College, Anhui University of Traditional Chinese Medicine, Hefei 230038, China.
Zhongguo Zhong Yao Za Zhi. 2013 Jul;38(14):2348-53.
To study the preventive and therapeutic effects of total saponin of Dioscorea (TSD) on chronic hyperuricemia, and its effect on urate transporter 1 (URAT1) in rats.
Ninety male rats were randomly divided into 6 groups: the normal group, the model group, TSD high-, medium- and low-dose (300, 100, 30 mg x kg(-1)) groups and the benzbromarone (10 mg x kg(-1)) group. Potassium oxonate and ethambutol were adopted to establish the chronic hyperuricemia model Since the third week, all the rats were intragastrically administered with drugs for 4 weeks, once a day, in order to determine their uric acid in serum and urine, uric acid excretion and xanthine oxidase (XOD). URAT1 mRNA and URAT1 protein expression in rat renal tubular cells were determined by RT-PCR and immunohistochemistry method respectively.
Serum uric acid level of the model group increased significantly, while uric acid excretion decreased, with high expressions of renal URAT1 mRNA and URAT1 protein. TSD could dose-dependently reduce the serum uric acid level of chronic hyperuricemia rats, increase the concentration of uric acid and uric acid excretion in urine, and reduce renal URAT1 mRNA and URAT1 protein expression. Its effects were similar with that of benzbromarone, but with no significant effect on XOD and urinary volume of chronic hyperuricemia rats.
TSD has an obvious effect of anti-hyperuricemia It may reduce the reabsorption of uric acid by inhibiting the high expression of rat renal URAT1.
研究穿山龙总皂苷（TSD）对慢性高尿酸血症的防治作用及其对大鼠尿酸转运蛋白1（URAT1）的影响。
将90只雄性大鼠随机分为6组：正常组、模型组、TSD高、中、低剂量（300、100、30mg·kg⁻¹）组和苯溴马隆（10mg·kg⁻¹）组。采用氧嗪酸钾和乙胺丁醇建立慢性高尿酸血症模型。自第3周起，所有大鼠每天灌胃给药1次，连续4周，以检测血清和尿液中的尿酸、尿酸排泄及黄嘌呤氧化酶（XOD）。分别采用RT-PCR和免疫组化法检测大鼠肾小管细胞中URAT1 mRNA和URAT1蛋白的表达。
模型组血清尿酸水平显著升高，尿酸排泄减少，肾URAT1 mRNA和URAT1蛋白高表达。TSD可剂量依赖性降低慢性高尿酸血症大鼠的血清尿酸水平，增加尿尿酸浓度和尿酸排泄，降低肾URAT1 mRNA和URAT1蛋白表达。其作用与苯溴马隆相似，但对慢性高尿酸血症大鼠的XOD和尿量无明显影响。
TSD具有明显的抗高尿酸血症作用，可能通过抑制大鼠肾URAT1的高表达减少尿酸重吸收。
