Chen Yan, Chen Xiao-Lin, Xiang Ting, Sun Bao-Guo, Luo Hao-Xuan, Liu Meng-Ting, Chen Ze-Xiong, Zhang Shi-Jun, Wang Chang-Jun
Department of Traditional Chinese Medicine, the First Affiliated Hospital of Sun Yat-sen University, Guangzhou, 510080, China.
Department of Liver Diseases, Traditional Chinese Medical Hospital of Guangdong Province, Guangzhou, 510120, China.
J Huazhong Univ Sci Technolog Med Sci. 2016 Apr;36(2):237-242. doi: 10.1007/s11596-016-1573-z. Epub 2016 Apr 13.
The aim of this study is to evaluate the efficacy of total saponins of Dioscorea (TSD), an extract of the Chinese herbal Bi Xie, on hyperuricemia and to elucidate the underlying mechanisms. The rat hyperuricemia model was established by administration of adenine. Thirty-two rats were randomly allocated into 4 groups: model group, low/high-dose TSD-treated groups, and allopurinol-treated group. Meanwhile, 8 rats were used as normal controls. Serum uric acid (UA), blood urea nitrogen (BUN), serum creatinine (Scr), and organic anion transporting polypeptide 1A1 (OATP1A1) levels were measured. Comparison between the model group and treatment (allopurinol and TSD) groups showed the serum UA levels were significantly decreased in treatment groups. TSD had similar effects to allopurinol. It was found that the OATP1A1 protein expression levels in treatment groups were higher than in model group and normal controls. And different from the allopurinol-treated groups, TSD-treated group had elevated OATP1A1 expression levels in the stomach, liver, small intestine and large intestine tissues. It was suggested that TSD may facilitate the excretion of UA and lower UA levels by up-regulating OATP1A1 expression.
本研究旨在评估中药萆薢提取物薯蓣总皂苷（TSD）对高尿酸血症的疗效，并阐明其潜在机制。通过给予腺嘌呤建立大鼠高尿酸血症模型。将32只大鼠随机分为4组：模型组、低/高剂量TSD治疗组和别嘌醇治疗组。同时，8只大鼠作为正常对照。检测血清尿酸（UA）、血尿素氮（BUN）、血清肌酐（Scr）和有机阴离子转运多肽1A1（OATP1A1）水平。模型组与治疗（别嘌醇和TSD）组之间的比较显示，治疗组血清UA水平显著降低。TSD与别嘌醇具有相似的作用。发现治疗组中OATP1A1蛋白表达水平高于模型组和正常对照组。与别嘌醇治疗组不同，TSD治疗组胃、肝、小肠和大肠组织中OATP1A1表达水平升高。提示TSD可能通过上调OATP1A1表达促进UA排泄并降低UA水平。
