[重组酵母乙肝疫苗免疫蛋鸡所产鸡蛋中抗乙肝病毒IgY二级结构的圆二色色谱分析]
[Circular dichroism chromatography of secondary structures of anti-HBV IgY in eggs from hens immunized with recombinant yeast HBV vaccine].
作者信息
Fang Xixiu, Fang Yuan, Wang Dongmei, Qi Fugang
机构信息
Jiangsu Agri-animal Husbandry Vocational College, Taizhou 225300, China.
出版信息
Xi Bao Yu Fen Zi Mian Yi Xue Za Zhi. 2013 Nov;29(11):1205-7.
OBJECTIVE
To study the generation and structure of anti-HBV IgY in eggs from hens immunized with recombinant yeast HBV vaccine.
METHODS
From the hens immunized three times with the recombinant yeast HBV vaccine, the eggs were collected to extract anti-HBV IgY. After purified, the anti- HBV IgY was detected by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) and reverse phase high-performance liquid chromatography (RP-HPLC), and its secondary structure was examined by circular dichroism (CD) chromatography.
RESULTS
The relative molecular mass (Mr;) of anti-HBV IgY expressed by the recombinant yeast HBV vaccine on hens was 178 000. CD chromatography showed that the α-helix, β-fold, β-turn and random coil of anti-HBV IgY had 38, 0, 44 and 17 amino acids, respectively.
CONCLUSION
The anti-HBV IgY can be expressed in eggs from hens immunized with recombinant yeast HBV vaccine. The α-helix and β-turn are major secondary structures of anti-HBV IgY.
目的
研究用重组酵母乙肝疫苗免疫的母鸡所产鸡蛋中抗乙肝病毒IgY的产生及结构。
方法
从用重组酵母乙肝疫苗免疫三次的母鸡收集鸡蛋，提取抗乙肝病毒IgY。纯化后，通过十二烷基硫酸钠-聚丙烯酰胺凝胶电泳（SDS-PAGE）和反相高效液相色谱（RP-HPLC）检测抗乙肝病毒IgY，并通过圆二色（CD）色谱法检测其二级结构。
结果
重组酵母乙肝疫苗在母鸡体内表达的抗乙肝病毒IgY的相对分子质量（Mr）为178 000。CD色谱显示，抗乙肝病毒IgY的α-螺旋、β-折叠、β-转角和无规卷曲分别含有38、0、44和17个氨基酸。
结论
抗乙肝病毒IgY可在用重组酵母乙肝疫苗免疫的母鸡所产鸡蛋中表达。α-螺旋和β-转角是抗乙肝病毒IgY的主要二级结构。
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