Instituto de Agroquímica y Tecnología de Alimentos (CSIC), Avenue Agustín Escardino 7, 46980 Paterna, Valencia, Spain.
Meat Sci. 2014 Feb;96(2 Pt A):757-61. doi: 10.1016/j.meatsci.2013.09.014. Epub 2013 Sep 25.
Dipeptidyl peptidase IV (DPP-IV) inhibitors are promising new therapies for type 2 diabetes. The aim of this study was to assay DPP-IV inhibitory peptides that can be present in a water soluble extract of Spanish dry-cured ham. Such an extract was fractionated by size-exclusion chromatography and the in vitro DPP-IV inhibitory activity determined in each collected fraction. Then, several peptides previously identified in dry-cured ham extracts or known to be products of DPP IV action were synthesised and assayed for DPP-IV inhibition. Peptides KA and AAATP showed the strongest DPP-IV inhibitory activity, with IC50 values of 6.27 mM and 6.47 mM, respectively. Dipeptides AA, GP, PL, and carnosine, as well as peptides AAAAG, ALGGA, and LVSGM were also DPP-IV inhibitors, although at a lower degree. These findings suggest the potential of Spanish dry-cured ham as a natural precursor of DPP-IV inhibitory peptides. These biopeptides could also be used as ingredients for functional foods or pharmaceutical products against type 2 diabetes.
二肽基肽酶 IV（DPP-IV）抑制剂是治疗 2 型糖尿病的有前途的新疗法。本研究旨在测定可存在于西班牙干腌火腿水溶性提取物中的 DPP-IV 抑制肽。该提取物通过大小排阻色谱进行分级，并在每个收集的级分中测定体外 DPP-IV 抑制活性。然后，合成了先前在干腌火腿提取物中鉴定出或已知是 DPP IV 作用产物的几种肽，并测定了它们对 DPP-IV 的抑制作用。KA 和 AAATP 肽表现出最强的 DPP-IV 抑制活性，IC50 值分别为 6.27 mM 和 6.47 mM。二肽 AA、GP、PL 和肌肽，以及肽 AAAAG、ALGGA 和 LVSGM 也是 DPP-IV 抑制剂，尽管抑制程度较低。这些发现表明西班牙干腌火腿可能是 DPP-IV 抑制肽的天然前体。这些生物活性肽也可以用作功能性食品或治疗 2 型糖尿病的药物产品的成分。
