用于构建嗜热栖热放线菌HTA426 DNA文库的三元缀合系统。
A ternary conjugation system for the construction of DNA libraries for Geobacillus kaustophilus HTA426.
作者信息
Suzuki Hirokazu, Wada Keisuke, Furukawa Megumi, Doi Katsumi, Ohshima Toshihisa
机构信息
Functional Genomics of Extremophiles, Faculty of Agriculture, Graduate School, Kyushu University.
出版信息
Biosci Biotechnol Biochem. 2013;77(11):2316-8. doi: 10.1271/bbb.130492. Epub 2013 Nov 7.
This report describes efficient plasmid uptake by the thermophile Geobacillus kaustophilus HTA426 by means of a ternary conjugation system, which was used to construct thermophile DNA libraries for G. kaustophilus and to identify the genes for orotidine-5'-phosphate decarboxylase by in vivo functional screening. The results indicate that the conjugation system is useful in constructing G. kaustophilus libraries, which are practical in identifying thermophile genes.
本报告描述了嗜热栖热放线菌HTA426通过三元接合系统有效摄取质粒的过程，该系统用于构建嗜热栖热放线菌的嗜热菌DNA文库，并通过体内功能筛选鉴定乳清苷-5'-磷酸脱羧酶的基因。结果表明，该接合系统可用于构建嗜热栖热放线菌文库，这对于鉴定嗜热菌基因具有实际意义。
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