Suzuki Hirokazu, Wada Keisuke, Furukawa Megumi, Doi Katsumi, Ohshima Toshihisa
Functional Genomics of Extremophiles, Faculty of Agriculture, Graduate School, Kyushu University.
Biosci Biotechnol Biochem. 2013;77(11):2316-8. doi: 10.1271/bbb.130492. Epub 2013 Nov 7.
This report describes efficient plasmid uptake by the thermophile Geobacillus kaustophilus HTA426 by means of a ternary conjugation system, which was used to construct thermophile DNA libraries for G. kaustophilus and to identify the genes for orotidine-5'-phosphate decarboxylase by in vivo functional screening. The results indicate that the conjugation system is useful in constructing G. kaustophilus libraries, which are practical in identifying thermophile genes.
本报告描述了嗜热栖热放线菌HTA426通过三元接合系统有效摄取质粒的过程，该系统用于构建嗜热栖热放线菌的嗜热菌DNA文库，并通过体内功能筛选鉴定乳清苷-5'-磷酸脱羧酶的基因。结果表明，该接合系统可用于构建嗜热栖热放线菌文库，这对于鉴定嗜热菌基因具有实际意义。
