早期生活铅暴露对幼鼠海马胰岛素样生长因子 1 和 2（IGF1、IGF2）表达的影响。
The effects of early life lead exposure on the expression of insulin-like growth factor 1 and 2 (IGF1, IGF2) in the hippocampus of mouse pups.
机构信息
College of Food Science and Technology, Henan Agriculture University, Zhengzhou 450002, China.
Public Health College, Zhengzhou University, Zhengzhou 450001, China.
出版信息
Food Chem Toxicol. 2014 Jan;63:48-52. doi: 10.1016/j.fct.2013.10.037. Epub 2013 Nov 5.
The present study was undertaken to investigate the effects of maternal lead exposure on expression of IGF1 and IGF2 in the hippocampus of mice offspring. Lead exposure initiated from beginning of gestation to weaning. Lead acetate administered in drinking solutions was dissolved in distilled deionized water at the concentrations of 0.1%, 0.5% and 1% groups respectively. On the 21st postnatal day, the learning and memory ability was tested by Water Maze test and the Pb levels were also determined by graphite furnace atomic absorption spectrometry. The expression of IGF1 and IGF2 in hippocampus was examined by immunohistochemistry and western blotting. The lead levels in blood and hippocampus of all lead exposure groups were significantly higher than that of the control group (P<0.05). In Water Maze test, the performances of 0.5% and 1% lead exposure groupswere worse than that of the control group (P<0.05). The expression of IGF1 and IGF2 was decreased in lead exposed groups than that of the control group (P<0.05). The low expression of IGF1 and IGF2 in the hippocampus of pups may contribute to the impairment of learning and memory associated with maternal Pb exposure.
本研究旨在探讨母鼠铅暴露对其子代海马 IGF1 和 IGF2 表达的影响。铅暴露始于妊娠开始至断奶。醋酸铅以 0.1%、0.5%和 1%的浓度溶解在蒸馏去离子水中，分别添加到饮水中。在出生后第 21 天，通过水迷宫试验测试学习和记忆能力，石墨炉原子吸收光谱法测定铅含量。通过免疫组织化学和 Western blot 检测海马 IGF1 和 IGF2 的表达。所有铅暴露组的血液和海马中的铅含量均明显高于对照组（P<0.05）。在水迷宫试验中，0.5%和 1%铅暴露组的表现均差于对照组（P<0.05）。与对照组相比，铅暴露组 IGF1 和 IGF2 的表达减少（P<0.05）。幼鼠海马中 IGF1 和 IGF2 的低表达可能导致与母鼠 Pb 暴露相关的学习和记忆损伤。
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