利用核糖体展示技术在导电聚合物上进行肽适体的体外选择。
In vitro selection of peptide aptamers using a ribosome display for a conducting polymer.
机构信息
Nano Medical Engineering Laboratory, RIKEN, 2-1 Hirosawa, Wako-shi, Saitama 351-0198, Japan; Emergent Bioengineering Materials Research Team, RIKEN Center for Emergent Matter Science, 2-1 Hirosawa, Wako-shi, Saitama 351-0198, Japan; Department of Biological Information, Graduate School of Bioscience and Biotechnology, Tokyo Institute of Technology, 1259 Nagatsuda, Midori-ku, Yokohama-shi, Kanagawa 226-8501, Japan.
Nano Medical Engineering Laboratory, RIKEN, 2-1 Hirosawa, Wako-shi, Saitama 351-0198, Japan; Emergent Bioengineering Materials Research Team, RIKEN Center for Emergent Matter Science, 2-1 Hirosawa, Wako-shi, Saitama 351-0198, Japan.
出版信息
J Biosci Bioeng. 2014 Apr;117(4):501-3. doi: 10.1016/j.jbiosc.2013.10.005. Epub 2013 Nov 5.
Ribosome display was used to select peptide aptamers from a random library composed of hydrophilic amino acids for a conducting polymer, poly(3-hexylthiophene-2,5-diyl). Binding of aptamers was measured by quartz crystal microbalance, and the secondary structure of the peptide was investigated by circular dichroism.
核糖体展示技术用于从由亲水氨基酸组成的导电聚合物聚(3-己基噻吩-2,5-二基)的随机文库中筛选肽适体。通过石英晶体微天平测量适体的结合，通过圆二色性研究肽的二级结构。
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