Li Ruowei, Kang Guangbo, Hu Min, Huang He
Department of Biochemical Engineering, School of Chemical Engineering & Technology, Tianjin University, Tianjin, 300350, People's Republic of China.
Key Laboratory of Systems Bioengineering (Ministry of Education), Tianjin University, Tianjin, 300072, People's Republic of China.
Mol Biotechnol. 2019 Jan;61(1):60-71. doi: 10.1007/s12033-018-0133-0.
Ribosome display is a powerful engineering research tool for the high-throughput selection of peptides or proteins, which results in the generation of high-performance binders against nearly any antigen of interest. As a cell-free display system, ribosome display has been well developed with many outstanding achievements for over 20 years. Compared with other related display techniques, ribosome display shows unique advantages and development prospects. This tool has been successfully exploited for the selection of functional and specific binders in vitro. This review provides a comprehensive survey of the applications of ribosome display in screening or evolving functional proteins as well as in diagnostics and therapeutics. Previous papers on ribosome display failed to comprehensively review evolutionary strategies for proteins. In the present paper, we review all existing evolutionary strategies that have been combined with ribosome display. We also discuss shortcomings, improvement strategies, and research tendency.
核糖体展示是一种用于高通量筛选肽或蛋白质的强大工程研究工具，可产生针对几乎任何感兴趣抗原的高性能结合物。作为一种无细胞展示系统，核糖体展示在20多年来得到了很好的发展并取得了许多杰出成就。与其他相关展示技术相比，核糖体展示具有独特的优势和发展前景。该工具已成功用于体外筛选功能性和特异性结合物。本文全面综述了核糖体展示在筛选或进化功能蛋白以及诊断和治疗方面的应用。以往关于核糖体展示的论文未能全面综述蛋白质的进化策略。在本文中，我们综述了所有与核糖体展示相结合的现有进化策略。我们还讨论了缺点、改进策略和研究趋势。
