Osawa Ayako, Iki Kanoko, Sandmann Gerhard, Shindo Kazutoshi
Department of Food and Nutrition, Japan Women's University.
J Oleo Sci. 2013;62(11):955-60. doi: 10.5650/jos.62.955.
We succeeded in purifying the carotenoid produced by Bacillus firmus GB1 using chromatography on polystyrenic synthetic adsorbents, and identified it as 4,4'-diapolycopene-4,4'-dioic acid by HRESI-MS and NMR analyses of it and its dimethylester. In addition, we clarified the singlet oxygen (¹O₂) quenching activities of 4,4'-diapolycopene-4,4'-dioic acid and its methyl esters (mono and dimethyl ester). Their IC₅₀ values were 5.8 μM, 6.0 μM, and 6.2 μM, respectively. To our knowledge, this is the first report concerning the isolation and antioxidative activity of 4,4'-diapolycopene-4,4'-dioic acid, a product of the C30 carotenoid biosynthesis pathway.
我们通过在聚苯乙烯合成吸附剂上进行色谱分离，成功纯化了坚强芽孢杆菌GB1产生的类胡萝卜素，并通过对其及其二甲基酯进行高分辨电喷雾电离质谱（HRESI-MS）和核磁共振（NMR）分析，将其鉴定为4,4'-二脱辅基多聚番茄红素-4,4'-二酸。此外，我们还阐明了4,4'-二脱辅基多聚番茄红素-4,4'-二酸及其甲酯（单甲酯和二甲基酯）的单线态氧（¹O₂）猝灭活性。它们的半数抑制浓度（IC₅₀）值分别为5.8 μM、6.0 μM和6.2 μM。据我们所知，这是关于C30类胡萝卜素生物合成途径产物4,4'-二脱辅基多聚番茄红素-4,4'-二酸的分离及其抗氧化活性的首次报道。
