Characterization of C carotenoid and identification of its biosynthetic gene cluster in AM1.

species, the representative bacteria distributed in phyllosphere region of plants, often synthesize carotenoids to resist harmful UV radiations. is known to produce a carotenoid pigment and recent research revealed that this carotenoid has a C backbone. However, its exact structure remains unknown. In the present study, the carotenoid produced by AM1 was isolated and its structure was determined as 4-[2--11-octadecenoyl-β-glucopyranosyl]-4,4'-diapolycopenedioc acid (), a glycosylated C carotenoid. Furthermore, the genes related to the C carotenoid synthesis were investigated. Squalene, the precursor of the C carotenoid, is synthesized by the co-occurrence of META1p1815, META1p1816 and META1p1817. Further overexpression of the genes related to squalene synthesis improved the titer of carotenoid . By using gene deletion and gene complementation experiments, the glycosyltransferase META1p3663 and acyltransferase META1p3664 were firstly confirmed to catalyze the tailoring steps from 4,4'-diapolycopene-4,4'-dioic acid to carotenoid . In conclusion, the structure and biosynthetic genes of carotenoid produced by AM1 were firstly characterized in this work, which shed lights on engineering AM1 for producing carotenoid in high yield.