Department of Agricultural Sciences, University of Bristol, AFRC Institute of Arable Crops Research, Long Ashton Research Station, BS18 9AF, Bristol, UK.
Planta. 1989 Dec;180(1):16-23. doi: 10.1007/BF02411405.
When pea (Pisum sativum L.) embryos were cultured on low osmotica, with or without added abscisic acid (ABA), there was very little change in the total mRNA translation products resolved by one-dimensional sodium dodecyl sulphate-polyacrylamide gel electrophoresis (SDS-PAGE). The only marked alteration was an increase in production of two low-molecular-weight proteins. The purification and partial characterisation of these two ABA-responsive seed proteins (ABR17 and ABR18) is described. Both proteins were purified to homoeneity, as judged by SDS-PAGE, from embryos cultured in the presence of ABA. Antisera were raised against both proteins. Each serum cross-reacted with the other protein, indicating that the proteins are closely related. Their apparent molecular masses (Mrs) were estimated to be 17200 (ABR17) and 18100 (ABR18) by SDS-PAGE, and 26000 by gel filtration. Both proteins were heterogeneous on isoelectric focusing. Neither protein was detected (by immunoblotting or immunoprecipitation of cell-free translation products) in embryos grown in vivo at early to mid-development stages but both were present in embryos late in development. These proteins appear to be produced late in seed development but are capable of being induced early in development by culturing embryos in vitro and are markedly enhanced by ABA.
当豌豆（Pisum sativum L.）胚胎在低渗条件下培养时，无论是否添加脱落酸（ABA），通过一维十二烷基硫酸钠-聚丙烯酰胺凝胶电泳（SDS-PAGE）解析的总 mRNA 翻译产物几乎没有变化。唯一明显的变化是两种低分子量蛋白质的产量增加。这两种 ABA 响应种子蛋白（ABR17 和 ABR18）的纯化和部分特性描述如下。两种蛋白质均通过 SDS-PAGE 从在 ABA 存在下培养的胚胎中纯化至均一性。针对两种蛋白质制备了抗血清。每种血清均与另一种蛋白质发生交叉反应，表明这些蛋白质密切相关。它们的表观分子量（Mr）通过 SDS-PAGE 估计为 17200（ABR17）和 18100（ABR18），通过凝胶过滤为 26000。两种蛋白质在等电聚焦上均呈异质。在早期至中期发育阶段体内生长的胚胎中，通过免疫印迹或无细胞翻译产物的免疫沉淀均未检测到（ABR17）和 18100（ABR18），但在发育后期的胚胎中均存在。这些蛋白质似乎在种子发育后期产生，但能够通过体外培养胚胎早期诱导，并被 ABA 显著增强。
