低温对马铃薯块茎磷酸果糖激酶活性的影响。

Effect of low temperature on the activity of phosphofructokinase from potato tubers.

机构信息

Department of Biochemistry and Physiology, AFRC Institute of Arable Crops Research, Rothamsted Experimental Station, AL5 2JQ, Harpenden, Herts, UK.

出版信息

Planta. 1990 Mar;180(4):613-6. doi: 10.1007/BF02411461.

DOI:10.1007/BF02411461

PMID:24202108

Abstract

The aim of this work was to compare the coldlability of phosphofructokinase (EC 2.7.1.11) from tubers of potato cultivars (cvs.) known to differ in their propensity to accumulate sugars at low temperature. When stored at 4°C for six weeks, the sugar content of tubers ofSolanum tuberosum L. cv. Record doubled whereas the amount of sugar in tubers of cv. Brodick and an advanced breeding clone (13676) decreased slightly. Tubers from each line contained four forms of phophofructokinase. Over the range 12°-16°C the temperature coefficients of the four forms of phosphofructokinase from cvs. Record and Brodick were similar. In cv. Record the temperature coefficients of three of the enzyme forms were significantly higher at 2°-6°C than at 12°-16°C, whereas those from cv. Brodick were unchanged. These results are consistent with the proposal that inactivation of phosphofructokinase at low temperature results in the accumulation of hexose phosphates leading to increased sucrose synthesis.

摘要

本工作旨在比较不同低温糖积累倾向的马铃薯品种（品系）块茎中磷酸果糖激酶（EC 2.7.1.11）的冷稳定性。在 4°C 下贮藏 6 周后，Solanum tuberosum L. cv. Record 块茎中的糖含量增加了一倍，而 cv. Brodick 和一个先进的育种种群（13676）的糖含量略有下降。每个品系的块茎中都含有四种形式的磷酸果糖激酶。在 12°-16°C 的范围内，来自 cv. Record 和 Brodick 的四种磷酸果糖激酶的温度系数相似。在 cv. Record 中，三种酶形式的温度系数在 2°-6°C 时明显高于 12°-16°C，而 cv. Brodick 的则保持不变。这些结果与低温下磷酸果糖激酶失活导致己糖磷酸积累增加从而导致蔗糖合成增加的假说一致。