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通过体细胞胚水平的体外选择生产耐盐芥菜植物。

Production of sodium-chloride-tolerant Brassica juncea plants by in vitro selection at the somatic embryo level.

机构信息

Biotechnology Center, Indian Agricultural Research Institute, 110012, New Delhi, India.

出版信息

Theor Appl Genet. 1991 Dec;83(2):233-7. doi: 10.1007/BF00226256.

Abstract

Somatic embryos, developed from hypocotyl segments of light-grown seedlings of Brassica juncea cv RLM198, were subjected to selection at varying concentrations of sodium chloride (NaCl). Plants were developed from proliferated somatic embryos selected on NaCl-containing medium. The selections were characterized for salt tolerance, esterase isozyme pattern, and proline accumulation. It has been found that: (i) selected tolerant lines showed better root growth, shoot growth, and fresh weight accumulation on salt-containing medium when compared to the control; (ii) salt tolerance was transmitted to the next generation in seed progeny of tolerant plants grown in the absence of exposure to salt; (iii) both the starting material and the tolerant selections accumulated proline, even when grown in salt-free medium. On salt-containing medium, however, the differences in accumulated proline between the control and tolerant lines became more pronounced, and (iv) the patterns of esterase isozymes of two tolerant selections were similar but distinctly different from that of the parental control.

摘要

体细胞胚,由萝卜品种 RLM198 的光培养子叶切段发育而来,在不同浓度的氯化钠(NaCl)下进行选择。从含有 NaCl 的培养基上选择的增殖体细胞胚发育为植株。对这些选择品系进行耐盐性、酯酶同工酶模式和脯氨酸积累的特征分析。结果表明:(i)与对照相比,耐盐系在含盐水培基上的根生长、茎生长和鲜重积累更好;(ii)在无盐暴露条件下生长的耐盐植株的种子后代中,耐盐性被传递到下一代;(iii)起始材料和耐盐选择品系在无盐培养基中均积累脯氨酸;(iv)然而,在含盐水培基上,耐盐系和对照系之间积累的脯氨酸差异更加明显,(iv)两种耐盐选择品系的酯酶同工酶模式相似,但与亲本对照明显不同。

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