Department of Biology, University of North Carolina, 27599-3280, Chapel Hill, NC, USA.
Plant Cell Rep. 1992 Jun;11(5-6):248-52. doi: 10.1007/BF00235075.
Immature maize spikelets have been successfully grown in vitro. Culture conditions were refined to maximize development of normal pollen grains. Kinetin was not required for normal development, in contrast to the absolute requirement for this plant growth regulator for in vitro tassel development. Development occured in all stages sampled, from premeiosis to postvacuolation, and there was no lag in progression through the various stages of development as compared to greenhouse-grown material. Cultured spikelets produced pollen that appeared morphologically normal, accumulated starch and had the normal two sperm nuclei and single vegetative nucleus.
已成功在体外培养不成熟的玉米小穗。培养条件经过优化，以最大限度地促进正常花粉粒的发育。与体外穗发育绝对需要这种植物生长调节剂相反，细胞分裂素不是正常发育所必需的。在取样的所有阶段都发生了发育，从减数分裂前到液泡化后，与在温室中生长的材料相比，各个发育阶段的进展没有滞后。培养的小穗产生的花粉在形态上看起来正常，积累淀粉，具有正常的两个精子核和一个营养核。
