在含有腐胺、精氨酸和鸟氨酸的条件下进行芋头的离体繁殖：I. 从主芽顶和腋芽再生小植株。

In vitro propagation of taro, with spermine, arginine, and ornithine : I. Plantlet regeneration from primary shoot apices and axillary buds.

机构信息

Department of Botany, University of Malaya, 59100, Kuala Lumpur, Malaysia.

出版信息

Plant Cell Rep. 1992 Jun;11(5-6):290-4. doi: 10.1007/BF00235084.

PMID:24203142

Abstract

In vitro growth and multiplication of taro [Colocasia esculenta var. antiquorum cv. Keladi Birah] was improved considerably, when primary shoot apices were cultured on two modifications of Linsmaier and Skoog [1965] medium, containing 5.5 mg 1(-1) naphthaleneacetic acid and 0.2 mg 1(-1) kinetin or 1.85 mg 1(-1) naphthaleneacetic acid and 2 mg 1(-1) kinetin and supplemented with 10(-4) or 10(-3) mol·1(-1) of polyamine spermine or either of the precursors of polyamine putrescine-arginine and ornithine. Plantlets were regenerated directly from primary shoot apices, axillary buds and protocorm-like bodies [PLB]. Frequency of plantlet regeneration, rate of development and growth in height of main plantlets were enhanced by the addition of arginine and ornithine to the media. Secondary plantlet formation from axillary buds and PLB were promoted by spermine and arginine respectively.

摘要

在离体条件下，芋（Colocasia esculenta var. antiquorum cv. Keladi Birah）的原球茎增殖和生长得到了极大的改善，当在两种改良的林斯梅尔和斯科格（1965）培养基上培养原球茎顶端时，培养基中分别含有 5.5 mg/L 的萘乙酸和 0.2 mg/L 的激动素或 1.85 mg/L 的萘乙酸和 2 mg/L 的激动素，并分别添加 10-4 或 10-3 mol/L 的多胺精胺或多胺的前体腐胺-精氨酸和鸟氨酸。植株可直接从原球茎顶端、腋芽和原球茎样体（PLB）再生。在培养基中添加精氨酸和鸟氨酸可提高植株再生的频率、发育速度和主植株的生长高度。通过添加精胺和精氨酸可分别促进腋芽和 PLB 的次生植株形成。