岩藻糖基GM1和具有NeuAcα2-6GalNAc连接（GD1e）的二唾液酸神经节苷脂在各种大鼠腹水肝癌细胞中的差异表达。
Differential expression of fucosyl GM1 and a disialoganglioside with a NeuAc alpha 2-6GalNAc linkage (GD1e) in various rat ascites hepatoma cells.
作者信息
Iwamori M, Sunada S, Ishihara E, Moki M, Fujimoto S, Nagai Y
出版信息
FEBS Lett. 1986 Mar 17;198(1):66-70. doi: 10.1016/0014-5793(86)81186-3.
A distinct difference in ganglioside composition among various rat ascites hepatomas and Yoshida sarcoma was observed on TLC-immunostaining with anti-fucosyl GM1 antibody, and chemical and enzymatic analyses. Yoshida sarcoma and ascites hepatomas, AH13, AH66F and AH66, but not the other 9 tumor cell lines investigated, specifically contained a disialoganglioside, NeuAc alpha 2-3Gal beta 1-3(NeuAc alpha 2-6)GalNAc beta 1-4Gal beta 1-4Glc beta 1-1ceramide (GD1e), whereas the 9 ascites hepatoma cells without GD1e contained fucosyl GM1. The differential expression of fucosyl GM1 and GD1e in various tumor cell lines indicates that different cell lineages express distinct metabolic pathways for gangliosides, and that the gangliosides are useful markers for distinguishing tumor cell lines.
在用抗岩藻糖基GM1抗体进行薄层层析免疫染色以及化学和酶分析时，观察到各种大鼠腹水肝癌和吉田肉瘤之间神经节苷脂组成存在明显差异。吉田肉瘤和腹水肝癌AH13、AH66F和AH66，而非所研究的其他9种肿瘤细胞系，特异性地含有一种二唾液酸神经节苷脂，即NeuAcα2-3Galβ1-3(NeuAcα2-6)GalNAcβ1-4Galβ1-4Glcβ1-1神经酰胺（GD1e），而不含GD1e的9种腹水肝癌细胞含有岩藻糖基GM1。各种肿瘤细胞系中岩藻糖基GM1和GD1e的差异表达表明，不同细胞谱系表达不同的神经节苷脂代谢途径，并且神经节苷脂是区分肿瘤细胞系的有用标志物。
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