大鼠腹水肝癌中GM1b神经节苷脂结构的进一步表征。

Further characterization of the structure of GM1b ganglioside from rat ascites hepatoma.

作者信息

Matsumoto M, Taki T, Samuelsson B, Pascher I, Hirabayashi Y, Li S C, Li Y T

出版信息

J Biol Chem. 1981 Sep 25;256(18):9737-41.

Abstract

A ganglioside named GM1b (Hirabayashi, Y., Taki, T., and Matsumoto, M. (1979) FEBS Let. 100, 253-257) with a sugar composition identical with that of GM1, II3 alpha NeuAc-GgOse4Cer (Gal beta 1 leads to 3GalNAc beta 1 leads to 4Gal(3 leads from 2 alpha NeuAc) beta 1 leads to 4G1c beta 1 leads to 1'ceramide), isolated from rat ascites hepatoma was further characterized. In contrast to GM1, the sialic acid in this ganglioside was susceptible to clostridial sialidase in the absence of bile salts. Based on the sequential enzymic hydrolysis, permethylation analysis and direct probe mass spectrometric analysis, the structure of this ganglioside is determined to be: NeuAc alpha 2 leads to 3Gal beta 1 leads to 3GalNAc beta 1 leads to 4Gal beta 1 leads to 4Glc leads to ceramide. The structure of this ganglioside is identical with that biosynthesized in vitro (Stoffyn, A., Stoffyn, P., and Yip, M. C. M. (1975) Biochim. Biophys. Acta 409, 97-103).

摘要

从大鼠腹水肝癌中分离出一种名为GM1b的神经节苷脂（Hirabayashi, Y., Taki, T., and Matsumoto, M. (1979) FEBS Let. 100, 253 - 257），其糖组成与GM1相同，即II3αNeuAc - GgOse4Cer（Galβ1→3GalNAcβ1→4Gal(3从2αNeuAc引出)β1→4Glcβ1→1'神经酰胺），并对其进行了进一步表征。与GM1不同的是，在没有胆汁盐的情况下，这种神经节苷脂中的唾液酸易受梭菌神经氨酸酶的作用。基于顺序酶促水解、全甲基化分析和直接探针质谱分析，确定这种神经节苷脂的结构为：NeuAcα2→3Galβ1→3GalNAcβ1→4Galβ1→4Glc→神经酰胺。这种神经节苷脂的结构与体外生物合成的结构相同（Stoffyn, A., Stoffyn, P., and Yip, M. C. M. (1975) Biochim. Biophys. Acta 409, 97 - 103）。