Svalander C, Eggertsen G, Olding L B
Histochemistry. 1986;84(1):81-5. doi: 10.1007/BF00493425.
Paraffin sections of formaldehyde-fixed renal biopsies were labeled for complement C3 by a polyclonal rabbit antibody to human complement C3, by the peroxidase-antiperoxidase complex (PAP) and the avidin-biotin peroxidase complex (ABC) techniques, respectively. All tissues had C3 deposits according to direct immunofluorescence on fresh frozen sections. Staining for muramidase was introduced as an intrinsic control for the degree of tissue proteolysis after the necessary trypsin digestion prior to the immunoenzyme labeling. The results indicated that even minute deposits of C3 could be detected in paraffin sections by the ABC method, which was more sensitive than the PAP technique; the ABC method allowed a maximal dilution of 1:2,400 of the primary antibody as compared to 1:800 for the PAP technique.
用抗人补体C3的兔多克隆抗体，分别通过过氧化物酶-抗过氧化物酶复合物（PAP）技术和抗生物素蛋白-生物素过氧化物酶复合物（ABC）技术，对甲醛固定的肾活检组织石蜡切片进行补体C3标记。根据新鲜冰冻切片上的直接免疫荧光检查，所有组织均有C3沉积。在免疫酶标记前进行必要的胰蛋白酶消化后，引入溶菌酶染色作为组织蛋白水解程度的内在对照。结果表明，通过ABC法可在石蜡切片中检测到即使是微量的C3沉积，该方法比PAP技术更敏感；与PAP技术的1:800相比，ABC法可将一抗最大稀释至1:2400。
