Wood G S, Warnke R
J Histochem Cytochem. 1981 Oct;29(10):1196-204. doi: 10.1177/29.10.7028859.
As biotin-avidin systems continue to be developed for applications involving single cells, cell suspensions, and especially tissue sections, the need arises for a method of blocking endogenous avidin-binding activity. One such method is described and its proposed mechanism is discussed. Utilizing this method, endogenous avidin-binding activity was detected and suppressed in selected human and murine tissues, thus facilitating the interpretation of specific immunohistochemical staining utilizing hybridoma monoclonal antibodies in a biotin-avidin-horseradish peroxidase detection system.
随着生物素-抗生物素蛋白系统不断地被开发用于涉及单细胞、细胞悬液，尤其是组织切片的应用中，就需要一种阻断内源性抗生物素蛋白结合活性的方法。本文描述了一种这样的方法，并讨论了其提出的作用机制。利用这种方法，在选定的人类和小鼠组织中检测并抑制了内源性抗生物素蛋白结合活性，从而便于在生物素-抗生物素蛋白-辣根过氧化物酶检测系统中利用杂交瘤单克隆抗体对特异性免疫组织化学染色进行解读。
