Yang H J, Sato T, Matsubara M, Tanishima T
Jpn J Ophthalmol. 1985;29(4):378-93.
Persistent bullous keratopathy was produced in one eye of a rabbit by anterior chamber irrigation with benzalkonium chloride solution (BAK). Corneal grafts were made from both eyes of another rabbit and they were transplanted to the eye with bullous keratopathy and the normal fellow eye, which served as the control. Subsequently, at intervals flat preparations of the endothelium of both eyes stained with silver nitrate were made. On panoramic photographs of the endothelium along one meridian of the cornea, the endothelial cell sizes were determined on both sides of the host-graft junction and at every 1 mm from the junction. In the control eyes the cell sizes increased, in the early postoperative period, near the host-graft junction both in the graft and in the host, and this increase gradually diminished by the end of the 6-month observation period. However, even after 6 months the mean cell size in both graft and host was slightly larger than the normal cell size. In the eye with bullous keratopathy, the cell size in the graft center increased rapidly and progressively. After 3 months the endothelial cells were seen in the host cornea and the cell sizes were considerably larger than those in the control cornea. After 6 months the cells were seen even in the periphery of the host cornea. In three sets of experiments, bullous keratopathy was produced in one eye of a male rabbit and the graft from a female rabbit was transplanted. Four months later, the detection frequency of the sex chromatin stained with acetic orcein was similar between the cells in the graft and in the host bullous cornea, indicating that the cells in the host had migrated from the graft.
用苯扎氯铵溶液（BAK）前房冲洗法在一只兔的一只眼睛中诱发持续性大疱性角膜病变。取自另一只兔双眼的角膜移植物被移植到患大疱性角膜病变的眼睛以及作为对照的正常对侧眼。随后，每隔一段时间制作硝酸银染色的双眼内皮细胞平铺标本。在角膜一条子午线的内皮细胞全景照片上，在宿主 - 移植物连接处两侧以及距连接处每1毫米处测定内皮细胞大小。在对照眼中，术后早期，移植物和宿主中靠近宿主 - 移植物连接处的细胞大小增加，并且在6个月观察期结束时这种增加逐渐减少。然而，即使在6个月后，移植物和宿主中的平均细胞大小仍略大于正常细胞大小。在患大疱性角膜病变的眼中，移植物中心的细胞大小迅速且持续增加。3个月后，在宿主角膜中可见内皮细胞，其细胞大小明显大于对照角膜中的细胞大小。6个月后，甚至在宿主角膜周边也可见到细胞。在三组实验中，在一只雄性兔的一只眼睛中诱发大疱性角膜病变，并移植来自雌性兔的移植物。4个月后，用醋酸地衣红染色的性染色质在移植物和宿主大疱角膜中的细胞间检测频率相似，表明宿主中的细胞已从移植物迁移而来。
