Di Giulio A M, Borella F, Mantegazza P, Hong J S, Panozzo C, Zanoni R, Gorio A
Peptides. 1985;6 Suppl 3:249-56. doi: 10.1016/0196-9781(85)90382-1.
Substance P and Met-enkephalin were detected by radioimmunoassay and immunocytochemistry in the rat lumbar spinal cord. The sciatic nerve was lesioned by resecting a piece and the proximal stump was either ligated, for limiting neurite outgrowth, or intraperitoneally sutured, allowing the formation of a large neuroma. Ten days post lesioning both peptide levels dropped approximately 50% and the punctate immunoreactivity decreased in the dorsal horn. Lesioning both sciatic nerves did not accelerate nor increase the extent of peptide loss compared to monolateral lesions. Immunocytochemistry showed that after bilateral lesioning also the punctate immunoreactivity in the dorsal horn decreased less drastically. However, FRAP staining of the dorsal horn decreased according to the lesion paradigms, mono- and bilaterally with the same intensity. Therefore nerve lesions trigger the process, but the peptidergic loss seems intraspinally regulated. In addition, both kinds of abnormal neurite outgrowth similarly altered peptide levels and distribution in the spinal cord. Our data suggest that pain states related to peripheral nerve lesions may be due to opiate peptide loss rather than to neuroma.
采用放射免疫分析法和免疫细胞化学法在大鼠腰段脊髓中检测P物质和甲硫氨酸脑啡肽。通过切除一段坐骨神经造成损伤，近端残端要么结扎以限制神经突生长，要么进行腹腔内缝合以形成大的神经瘤。损伤后10天，两种肽水平均下降约50%，背角的点状免疫反应性降低。与单侧损伤相比，双侧坐骨神经损伤既没有加速也没有增加肽的丢失程度。免疫细胞化学显示，双侧损伤后背角的点状免疫反应性也没有那么剧烈地降低。然而，背角的荧光恢复能力（FRAP）染色根据损伤模式而降低，单侧和双侧损伤的强度相同。因此，神经损伤引发了这一过程，但肽能物质的丢失似乎是脊髓内调节的。此外，两种异常的神经突生长同样改变了脊髓中肽的水平和分布。我们的数据表明，与周围神经损伤相关的疼痛状态可能是由于阿片肽的丢失而不是神经瘤。
