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基于 RFLP 的黑麦(Secale cereale L.)1R 染色体遗传图谱。

RFLP-based genetic map of rye (Secale cereale L.) chromosome 1R.

机构信息

Cambridge Laboratory, Colney Lane, NR4 7UJ, Norwich, UK.

出版信息

Theor Appl Genet. 1991 Aug;82(2):174-8. doi: 10.1007/BF00226209.

Abstract

A map of chromosome 1R of rye was constructed using 16 molecular and biochemical loci. From long arm to short arm, known-function loci were placed in the order: XAdh - XLee - Glu-R1[Sec-3] - XPpdk-1R - XEm-1R-1 - XEm-1R-2 - Centromere - XNor-R1 -Gpi-R1 - XGli-R1 [Sec-1a] along with six anonymous genomic and cDNA clones from wheat. The map, which spans 106 cM with 12 loci clustered in a 15-cM region around the centromere, shows reasonably good agreement with previously published maps for the centromeric region, whereas the XNor-R1 - Gpi-R1 region gives a much larger distance than previously reported.

摘要

利用 16 个分子和生化标记物,构建了黑麦 1R 染色体的图谱。从长臂到短臂,已知功能的标记物依次为:XAdh- XLee- Glu-R1[Sec-3]-XPpdk-1R- XEm-1R-1- XEm-1R-2- 着丝粒- XNor-R1-Gpi-R1- XGli-R1[Sec-1a],同时还有来自小麦的 6 个匿名基因组和 cDNA 克隆。该图谱跨越 106cM,12 个标记物聚集在着丝粒周围 15cM 的区域内,与先前发表的着丝粒区域图谱相当吻合,而 XNor-R1-Gpi-R1 区域的距离则比之前报道的要大得多。

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