Division of Biological Sciences, Tucker Hall, University of Missouri, 65211, Columbia, MO, USA.
Theor Appl Genet. 1995 Oct;91(5):720-6. doi: 10.1007/BF00220949.
A genetic map of rye, Secale cereale L., chromosome 1R covering 247 cM was constructed utilizing 27 RFLP and four C-band markers, including terminal C-bands. Genetic mapping of C-bands and the centromere, and in situ hybridization of three RFLP clones, allowed for the integration of the genetic and cytological maps. Eight contact points between the genetic and cytological maps revealed variation in the recombination distance to cytological distance ratio ranging between 0.25 and 1.95, a 7.8-fold difference. Recombination was found to be highest in the satellite region of 1RS and lowest in the most distal region of 1RL.
利用 27 个 RFLP 和 4 个 C 带标记（包括端 C 带）构建了覆盖 247cM 的黑麦（Secale cereale L.）1R 染色体的遗传图谱。C 带和着丝粒的遗传作图以及 3 个 RFLP 克隆的原位杂交，使得遗传图谱和细胞学图谱得以整合。遗传图谱和细胞学图谱之间的 8 个接触点揭示了重组距离与细胞学距离比值的变化范围在 0.25 到 1.95 之间，相差 7.8 倍。在 1RS 的卫星区域发现了最高的重组率，而在 1RL 的最远端区域则发现了最低的重组率。
