固定在聚甲基丙烯酸缩水甘油酯包被的支持物上的抗体在饱和分析中的应用。

The use of antibody immobilized on supports coated with polyglycidyl methacrylate in saturation analysis.

作者信息

Rauch P, Marek M, Matas V, Vodrázka Z

出版信息

Anal Biochem. 1986 Feb 1;152(2):333-8. doi: 10.1016/0003-2697(86)90418-5.

Abstract

A new procedure for separation of free and bound ligand in saturation analysis (e.g., radioimmunoassay, competitive protein binding analysis) is presented. The antibody was immobilized on different carriers (glass rods, aluminium or polyethylene strips) covered with a thin layer of polyglycidyl methacrylate. The surface of the polymer had been activated by reaction with either ethylene diamine and glutaraldehyde or sulfuric acid and sodium periodate. The antibody was immobilized on this activated polymer by a covalent bond. The advantages of the presented separation methods are rapidity, simplicity, and conservation of the free and bound ligand equilibrium. A comparison with other separation techniques is carried out.

摘要

本文介绍了一种用于饱和分析（如放射免疫分析、竞争性蛋白结合分析）中游离配体和结合配体分离的新方法。抗体固定在覆盖有聚甲基丙烯酸缩水甘油酯薄层的不同载体（玻璃棒、铝条或聚乙烯条）上。聚合物表面通过与乙二胺和戊二醛或硫酸和高碘酸钠反应进行活化。抗体通过共价键固定在这种活化的聚合物上。所提出的分离方法的优点是快速、简单，并且能保持游离配体和结合配体的平衡。还与其他分离技术进行了比较。