Department of Animal and Poultry Science, College of Agriculture and Bioresources, University of Saskatchewan, 51 Campus Drive, Saskatoon, SK S7N 5A8, Canada; Animal Nutrition Institute, Sichuan Agriculture University, Sichuan 625014, China.
Animal Nutrition Institute, Sichuan Agriculture University, Sichuan 625014, China.
Spectrochim Acta A Mol Biomol Spectrosc. 2014;121:14-22. doi: 10.1016/j.saa.2013.10.029. Epub 2013 Oct 23.
An experiment was conducted to investigate the relationship of carbohydrates molecular spectral characteristics to rumen degradability of primary nutrients in Prairie feeds in dairy cattle. In total, 12 different types of feeds were selected, each type of feed was from three different source with total 37 samples. Six types of them were energy-sourced feeds and the others were protein-sourced feeds. The carbohydrates molecular spectral intensity of various functional groups were collected using Fourier transform infrared attenuated total reflectance (ATR-FT/IR) spectroscopy. In the in situ study, the results showed that the rumen digestibility and digestible fractions of primary nutrients (DM, OM, NCP, and CP) were significantly different (P<0.05) among the feeds. The spectral bands features were significantly different (P<0.05) among the feeds. Spectral intensities of A_Cell, H_1415 and H_1370 were weakly positively correlated with in situ rumen digestibility and digestible fractions of DM, OM and NCP. Spectral intensities of H_1150, H_1015, A_1, and A_3 were weakly negatively associated with in situ rumen degradation of CP. Spectral intensities of A_1240 and H_1240, mainly associated with cellulosic compounds, were correlated with rumen CP degradation. The multiple regression analysis demonstrated that the spectral intensities of A_3 and H_1415 played the most important role and could be used as a potential tool to predict rumen protein degradation of feeds in dairy cattle. In conclusion, this study showed that the carbohydrates as a whole have an effect on protein rumen degradation, rather than cellulose alone, indicating carbohydrate-protein matrix structure impact protein utilization in dairy cattle. The non-invasive molecular spectral technique (ATR-FT/IR) could be used as a rapid potential tool to predict rumen protein degradation of feedstuffs by using molecular spectral bands intensities in carbohydrate fingerprint region.
进行了一项实验，以研究碳水化合物分子光谱特征与奶牛草原饲料中主要营养素瘤胃降解率的关系。总共选择了 12 种不同类型的饲料，每种类型的饲料来自三个不同的来源，共 37 个样本。其中 6 种是能量饲料，其余是蛋白质饲料。使用傅里叶变换衰减全反射（ATR-FT/IR）光谱法收集各种官能团的碳水化合物分子光谱强度。在体内研究中，结果表明，饲料中主要营养素（DM、OM、NCP 和 CP）的瘤胃消化率和可消化部分有显著差异（P<0.05）。饲料之间的光谱带特征有显著差异（P<0.05）。A_Cell、H_1415 和 H_1370 的光谱强度与 DM、OM 和 NCP 的体内瘤胃消化率和可消化部分呈弱正相关。H_1150、H_1015、A_1 和 A_3 的光谱强度与 CP 的体内瘤胃降解呈弱负相关。A_1240 和 H_1240 的光谱强度主要与纤维素化合物有关，与瘤胃 CP 降解有关。多元回归分析表明，A_3 和 H_1415 的光谱强度起着最重要的作用，可以作为预测奶牛饲料瘤胃蛋白降解的潜在工具。总之，本研究表明碳水化合物整体对蛋白质瘤胃降解有影响，而不仅仅是纤维素，表明碳水化合物-蛋白质基质结构影响奶牛对蛋白质的利用。非侵入性分子光谱技术（ATR-FT/IR）可以作为一种快速潜在工具，通过使用碳水化合物指纹区域的分子光谱带强度来预测饲料的瘤胃蛋白降解。
