Du Weiping, Ye Wenjing, Chen Minghui, Li Dong, Jing Xuean
Department of Center for Disease Prevention and Control of Tai'an City, Tai'an 271000,China.
Wei Sheng Yan Jiu. 2013 Sep;42(5):770-6.
To explore the polymorphism of interferon-gamma (IFN-gamma) and interleukin-10 (IL-10) with susceptibility to esophageal cancer (EC), as well as the interaction between the environmental risk factors and the polymorphism of IFN-gamma or IL- 10. To explore the interaction between the polymorphism of IFN-gamma and IL-10.
According to the case-control design, 120 EC cases and 122 controls were examined. PCR technology was used to determine the genotypes of IFN-gamma + A874T and IL-10 -A1082G. Indicators of quantitative analysis for interaction between IFN-gamma and IL-10, and parts of environmental factors were calculated by crossover analysis multiplicative mode.
Allele frequencies of T of IFN-gamma + A874T was 37.92% in case group higher than 28.7% in control group (chi2 = 4.6414, P = 0.0312). Compared with IFN-gamma + 874AA genotype, OR of IFN-gamma + 874AT genotype was 1.729 (95% CI 1.015-2.947), OR of IFN-gamma + 874TT genotype was 2.923 (95% CI 1.227-5.214) and OR of IFN-gamma + 874AT + TT genotype was 1.821 (95% CI 1.081-3.069). There was synergistic action between IFN-gamma + 874AT + TT and drink index, moldy food and regular diet habit, their S respectively were 1.909, 4.154 and 5.026. The interaction between IFN-gamma + 874AT + TT and IL-10 -1082AG + GG was not increased the risk of EC OR = 2.342 (95% CI 0.975-5.626).
The IFN-gamma + 874AT genotype might be a risk factor to EC. T allele gene may be heredity predisposing gene of EC. IFN-gamma + 874 AT + TT genotype as the corresponding reduction of the environmental risk factors can reduce the incidence of esophageal cancer.
探讨干扰素-γ（IFN-γ）和白细胞介素-10（IL-10）基因多态性与食管癌（EC）易感性的关系，以及环境危险因素与IFN-γ或IL-10基因多态性之间的相互作用。探讨IFN-γ和IL-10基因多态性之间的相互作用。
采用病例对照研究设计，检测120例EC患者和122例对照者。应用聚合酶链反应（PCR）技术检测IFN-γ +A874T和IL-10 -A1082G的基因型。采用交叉分析乘法模型计算IFN-γ与IL-10及部分环境因素之间相互作用的定量分析指标。
病例组IFN-γ +A874T位点T等位基因频率为37.92%，高于对照组的28.7%（χ2 = 4.6414，P = 0.0312）。与IFN-γ +A874AA基因型相比，IFN-γ +A874AT基因型的比值比（OR）为1.729（95%可信区间1.015-2.947），IFN-γ +A874TT基因型的OR为2.923（95%可信区间1.227-5.214），IFN-γ +A874AT+TT基因型的OR为1.821（95%可信区间1.081-3.069）。IFN-γ +A874AT+TT与饮酒指数、霉变食物及规律饮食习惯之间存在协同作用，其协同指数（S）分别为1.909、4.154和5.026。IFN-γ +A874AT+TT与IL-10 -1082AG+GG之间的相互作用未增加EC的发病风险，OR = 2.342（95%可信区间0.975-5.626）。
IFN-γ +A874AT基因型可能是EC的危险因素。T等位基因可能是EC的遗传易感性基因。IFN-γ +A874AT+TT基因型联合相应环境危险因素的减少可降低食管癌的发病风险。
