Grady-Leopardi E F, Schwab M, Ablin A R, Rosenau W
Cancer Res. 1986 Jun;46(6):3196-9.
We studied N-myc oncogene expression in 13 human neuroectodermal tumors and one teratoma by in situ hybridization. In four of six neuroblastomas, there was increased N-myc expression (15 to 49% of the cells). Many of the primitive neuroblastic cells had an increase of N-myc RNA not observed in the larger, more differentiated cells. Two neuroblastomas matured to ganglioneuromas; no biopsies performed during this progression expressed increased N-myc RNA. Three ganglioneuroblastomas, two tumors presenting as ganglioneuromas, a cerebral neuroectodermal tumor, a neurofibrosarcoma, and the teratoma did not have increased N-myc expression. The data obtained by in situ hybridization correlated well with data obtained by blot analysis. Neuroblastomas/ganglioneuroblastomas with a favorable course did not have appreciable elevation of N-myc expression over 10 to 77 mo of follow-up; thus N-myc may not be involved in the maintenance of the neoplastic state. However, such tumors with a fatal outcome 2 to 14 mo after diagnosis usually had elevated N-myc expression. These findings suggest a relationship between elevated levels of N-myc RNA and poor prognosis.
我们通过原位杂交研究了13例人类神经外胚层肿瘤和1例畸胎瘤中的N - myc癌基因表达。在6例神经母细胞瘤中的4例中，N - myc表达增加（占细胞的15%至49%）。许多原始神经母细胞的N - myc RNA增加，而在较大、分化程度更高的细胞中未观察到这种增加。2例神经母细胞瘤成熟为神经节神经瘤；在这一进展过程中进行的活检均未显示N - myc RNA表达增加。3例神经节神经母细胞瘤、2例表现为神经节神经瘤的肿瘤、1例脑神经外胚层肿瘤、1例神经纤维肉瘤和1例畸胎瘤均未出现N - myc表达增加。原位杂交获得的数据与印迹分析获得的数据相关性良好。随访10至77个月，病程良好的神经母细胞瘤/神经节神经母细胞瘤N - myc表达没有明显升高；因此，N - myc可能不参与肿瘤状态的维持。然而，诊断后2至14个月出现致命结局的此类肿瘤通常N - myc表达升高。这些发现提示N - myc RNA水平升高与预后不良之间存在关联。
