College of Fishery, Guangdong Ocean University, Zhanjiang 524088, China; Guangdong Provincial Key Laboratory of Pathogenic Biology and Epidemiology for Aquatic Economic Animals, Zhanjiang 524088, China; Guangdong Key Laboratory of Control for Diseases of Aquatic Economic Animals, Zhanjiang 524088, China.
Guangdong Provincial Key Laboratory of Pathogenic Biology and Epidemiology for Aquatic Economic Animals, Zhanjiang 524088, China; Guangdong Key Laboratory of Control for Diseases of Aquatic Economic Animals, Zhanjiang 524088, China; Zhongkai University of Agriculture and Engineering, Guangzhou 510225, China.
Fish Shellfish Immunol. 2014 Jan;36(1):223-8. doi: 10.1016/j.fsi.2013.11.003. Epub 2013 Nov 9.
Nedd4 binding protein 1 (N4BP1) has been identified as an interacting protein and a substrate of Nedd4 E3 ligase. However, the report about N4BP1's function is limit. In this study, a novel N4BP1 gene (CiN4BP1) was cloned from grass carp (Ctenopharyngodon idella). The full-length cDNA sequence of CiN4BP1 (3022 bp) included an open reading frame (ORF) of 2565 bp, which encoded a putative peptides of 854 amino acids containing one KH domain and one NYN domain. It was close homology (47% identify) to Oryzias latipes N4BP1. And mRNA expression of CiN4BP1 gene showed relatively high level in skin, gill, head kidney and spleen. After grass carp reovirus (GCRV) infection, CiN4BP1 was up-regulated in vivo and in vitro. Furthermore, overexpression of CiN4BP1 in CIK cells inhibited viral gene transcription. These data indicated that CiN4BP1 might play an important role in immune response to viral invasion.
Nedd4 结合蛋白 1（N4BP1）已被鉴定为 Nedd4 E3 连接酶的相互作用蛋白和底物。然而，关于 N4BP1 功能的报道有限。本研究从草鱼（Ctenopharyngodon idella）中克隆了一种新型 N4BP1 基因（CiN4BP1）。CiN4BP1 的全长 cDNA 序列（3022 bp）包含一个 2565 bp 的开放阅读框（ORF），编码一个假定的肽，由 854 个氨基酸组成，含有一个 KH 结构域和一个 NYN 结构域。它与 Oryzias latipes N4BP1 的同源性较高（47%的同一性）。CiN4BP1 基因的 mRNA 表达在皮肤、鳃、头肾和脾脏中水平较高。草鱼呼肠孤病毒（GCRV）感染后，CiN4BP1 在体内和体外均上调。此外，CIK 细胞中 CiN4BP1 的过表达抑制了病毒基因的转录。这些数据表明 CiN4BP1 可能在抗病毒入侵的免疫反应中发挥重要作用。
