Nagano Vegetable and Ornamental Crops Experiment Station, 2206 Oomuro, Matsushiro, 381-12, Nagano, Japan.
Plant Cell Rep. 1991 Feb;9(10):531-4. doi: 10.1007/BF00232325.
Somatic hybrids between cultivated lettuce (Lactuca sativa) and a wild species L. virosa were produced by protoplast electrofusion. Hybrid selection was based on inactivation of L. sativa with 20mM iodoacetamide for 15 min, and the inability of L. virosa protoplasts to divide in the culture conditions used. Protoplasts were cultured in agarose beads in a revised MS media. In all 71 calli were formed and 21 of them differentiated shoots on LS medium containing 0.1mg/l NAA and 0.2mg/l BA. Most regenerated plants exhibited intermediate morphology. These plants were confirmed as hybrids by isoenzyme analysis. The majority of somatic hybrids had 2n=4x=36 chromosomes, and had more vigorous growth than either parent. Hybrids had normal flower morphology, but all were sterile.
通过原生质体电融合，培育了生菜（Lactuca sativa）和野生物种 L. virosa 之间的体细胞杂种。杂种的选择基于用 20mM 碘乙酰胺处理 15 分钟使生菜失活，以及在使用的培养条件下 L. virosa 原生质体无法分裂。原生质体在琼脂糖珠中在改良的 MS 培养基中培养。总共形成了 71 个愈伤组织，其中 21 个在含有 0.1mg/l NAA 和 0.2mg/l BA 的 LS 培养基上分化出芽。大多数再生植株表现出中间形态。这些植物通过同工酶分析被确认为杂种。大多数体细胞杂种具有 2n=4x=36 条染色体，比亲本生长更旺盛。杂种具有正常的花形态，但都是不育的。
