Physiologie des Poissons, INRA, Campus de Beaulieu, 35042, Rennes, France.
Fish Physiol Biochem. 1990 Nov;8(6):485-95. doi: 10.1007/BF00003405.
The protein composition of seminal fluid, blood serum, sperm plasma membrane and flagellum of rainbow trout were analysed by SDS-polyacrylamide gel electrophoresis. Immunological identity between proteins of the 2 fluids and sperm components was studied using crossed immunoelectrophoresis, rocket immunoelectrophoresis and immunoblotting. Results indicate that many seminal proteins are antigenically-related to serum proteins, proteins of sperm origin are present in seminal fluid in varying amounts, depending on the animals and sampling time, and several serum-like seminal proteins are bound to spermatozoa.Lipoproteins were isolated from seminal fluid (mean level: 33 μg/ml) and characterized. They were identified as being HDL-like lipoproteins. A possible physiological role is proposed for these seminal lipoproteins.
采用 SDS-聚丙烯酰胺凝胶电泳分析了虹鳟精液、血清、精子质膜和鞭毛的蛋白质组成。通过交叉免疫电泳、火箭免疫电泳和免疫印迹研究了 2 种液体的蛋白质与精子成分之间的免疫同源性。结果表明，许多精液蛋白与血清蛋白具有抗原相关性，来源于精子的蛋白以不同的量存在于精液中，这取决于动物和采样时间，并且几种类似血清的精液蛋白与精子结合。从精液中分离出脂蛋白（平均水平：33μg/ml）并进行了鉴定。它们被鉴定为 HDL 样脂蛋白。提出了这些精液脂蛋白的可能生理作用。
