钙离子在光形态建成控制的黄化小麦（Triticum aestivum L.）原生质体膨胀中的作用。

The role of calcium ions in phytochrome-controlled swelling of etiolated wheat (Triticum aestivum L.) protoplasts.

机构信息

Department of Plant Physiological Research, Agricultural University, Generaal Foulkesweg 72, NL-6703 BW, Wageningen, The Netherlands.

出版信息

Planta. 1988 Apr;174(1):94-100. doi: 10.1007/BF00394879.

DOI:10.1007/BF00394879

PMID:24221423

Abstract

Protoplasts from dark-grown wheat (Triticum aestivum L.) maintained at a constant osmotic potential at 22°C, were found to swell upon red irradiation (R) and the effect was negated by subsequent far-red light (FR), indicating phytochrome involvement. Swelling only occurred when Ca(2+) ions were present in the surrounding medium, or were added within 10 min after R. Furthermore, Mg(2+), Ba(2+) or K(+) could not replace this requirement for Ca(2+). The presence of K(+) did not enhance the Ca(2+)-dependent swelling response. When the Ca(2+)-ionophore A 23187 was added to the medium, protoplasts swelled in the dark to the same extent as after R. Both the Ca(2+)-channelblocker Verapamil and La(3+) inhibited R-induced swelling. It is proposed that R causes the opening of Ca(2+)-channels in the plasma membrane. Boyle-van't Hoff analyses of protoplast volume after R and FR are consistent with the conclusion that R irradiation causes changes in membrane properties.

摘要

在 22°C 下，渗透压保持恒定的黑暗中生长的小麦（Triticum aestivum L.）原生质体在红光（R）照射下会发生膨胀，随后的远红光（FR）会使这种效应消除，表明参与了光敏色素。只有在周围介质中存在 Ca(2+)离子或在 R 后 10 分钟内添加 Ca(2+)离子时，才会发生肿胀。此外，Mg(2+)、Ba(2+)或 K(+)不能替代 Ca(2+)的这种需求。K(+)的存在并没有增强 Ca(2+)-依赖性肿胀反应。当将 Ca(2+)载体 A 23187 添加到培养基中时，原生质体在黑暗中也会像在 R 之后一样发生肿胀。Ca(2+)通道阻滞剂维拉帕米和 La(3+)均抑制 R 诱导的肿胀。因此，提出 R 导致质膜中 Ca(2+)通道的打开。R 和 FR 后原生质体体积的 Boyle-van't Hoff 分析与 R 照射导致膜性质变化的结论一致。