从两个甜椒品种的子叶和下胚轴切段体进行离体植物再生。

In vitro plant regeneration from cotyledon and hypocotyl segments in two bell pepper cultivars.

机构信息

Centro de Investigaciones Agrarias, Comunidad Autónoma de La Rioja, Apdo. 1056, Logroño, Spain.

出版信息

Plant Cell Rep. 1991 Oct;10(8):414-6. doi: 10.1007/BF00232614.

Abstract

In vitro plant regeneration has been obtained from Capsicum annuum cvs. Pico and Piquillo. Shootbuds were induced from hypocotyl and cotyledon segments after 15-20 days of culture on MS basal medium supplemented with IAA and BAP or Zeatin. Shoot-buds grew into rosettes that rooted in MS plus NAA (0.1 mg/l) and IBA (0.05 mg/l) after 15 days. The small plantlets were successfully transferred to pots with a mixture of peat and perlite and maintained under greenhouse conditions. Elongation took place when the plantlets were growing in the greenhouse.

摘要

已经从辣椒品种 Pico 和 Piquillo 中获得了体外植物再生。在 MS 基本培养基上添加 IAA 和 BAP 或 Zeatin 培养 15-20 天后，从下胚轴和子叶节诱导出芽。芽长成莲座叶丛，在 MS 中添加 NAA（0.1mg/L）和 IBA（0.05mg/L）15 天后生根。将小植株成功转移到泥炭和珍珠岩的混合物中，并在温室条件下进行维护。当植株在温室中生长时，会发生伸长。