Plant Biotechnology Institute, IBARAKI Agricultural Center, Iwama, 319-02, Nishi-Ibaraki, Japan.
Plant Cell Rep. 1993 Oct;12(12):676-80. doi: 10.1007/BF00233418.
Organogenesis of shoots of bell pepper (Capsicum annuum L.) was achieved in fourteen cultivars on Murashige and Skoog's medium (MS medium) supplemented only with 0.4% (w/v) Gellan gum (pH 5.8). Mature seeds of cv. Shinsakigake-2 were sown on filter paper that had been wetted with sterilized water and precultured for zero to five days in under 16 hr of light per day at 25 °C. Explants, consisting of the proximal part of the hypocotyl and the radicle, were excised from the seeds and formed adventitious buds around the cut surfaces of elongated hypocotyls after four weeks of culture. When explants were subcultured on MS medium, 57% of the explants that had produced adventitious buds extended shoots after an additional three weeks of culture. Shoots were rooted on MS medium after two further weeks of culture. Chromosome numbers of all 30 regenerated plants that weexamined were normal (2n=24). The morphology of the mature plants was also normal and they set normally shaped fruits with mature seeds. Regenerated whole plants were also obtained in the case of 13 other cultivars by applying this simple procedure.
甜椒（Capsicum annuum L.）芽的器官发生是在十四种品种的 MS 培养基（仅补充 0.4%（w/v）结冷胶，pH5.8）上实现的。先将 Shinsakigake-2 品种的成熟种子播在浸湿的无菌滤纸上，在 25°C、每天 16 小时光照下预培养零至五天。从种子中切取包括下胚轴和胚根近端部分的外植体，在经过四周的培养后，在外植体伸长下胚轴的切面上形成不定芽。当外植体在 MS 培养基上继代培养时，经过三周的培养后，有 57%产生不定芽的外植体延长出芽。再经过两周的培养，芽生根在 MS 培养基上。我们检查的所有 30 株再生植株的染色体数目均正常（2n=24）。成熟植株的形态也正常，它们结出形状正常、种子成熟的果实。通过应用这种简单的程序，也获得了 13 个其他品种的再生全株植物。
