支持细胞在精子发生中的作用：姥鲨睾丸模型。

Role of the sertoli cell in spermatogenesis: TheSqualus testis model.

机构信息

Department of Biology, Boston University, 2 Cummington Street, Boston, MA, 02215, USA.

出版信息

Fish Physiol Biochem. 1989 Jun;7(1-6):221-7. doi: 10.1007/BF00004710.

Abstract

In the dogfish sharkSqualus acanthias different germ cell stages are topographically segregated within the testis. Using this species we have developed methods for the isolation and culture of Sertoli cells from premeiotic, meiotic and post-meiotic stages of spermatogenesis and present preliminary evidence for stage-dependent variations in cell morphology and behavior, thymidine incorporation, protein synthesis and steroidogenesis. The goal of future studies is to determine how maturational changes are regulated in Sertoli cells and, in turn, to elucidate Sertoli cell-germ cell interactions.

摘要

在角鲨属的棘鲛中，不同的生殖细胞阶段在睾丸内是按位置分隔开的。我们利用这一物种，开发了从精原细胞发生的前减数分裂、减数分裂和减数分裂后阶段分离和培养支持细胞的方法，并初步证明了细胞形态和行为、胸腺嘧啶核苷掺入、蛋白质合成和类固醇生成方面存在与阶段相关的变化。未来研究的目标是确定支持细胞中的成熟变化是如何受到调节的，进而阐明支持细胞-生殖细胞的相互作用。