Department of botany, University of Edinburgh, King's Buildings, Mayfield Road, EH9 3JH, Edinburgh, UK.
Planta. 1988 Sep;175(3):412-6. doi: 10.1007/BF00396348.
Hemicellulose extracted from cell walls of suspension-cultured rose (Rosa "Paul's Scarlet") cells was digested with cellulase from Trichoderma viride. The quantitatively major oligosaccharide products, a nonasaccharide and a heptasaccharide derived from xyloglucan, were purified by gel permeation chromatography. The nonasaccharide was found to inhibit the 2,4-dichlorophenoxy-acetic-acid-induced elongation of etiolated pea (Pisum sativum) stem segments. This confirms an earlier report (York et al., 1984, Plant Physiol. 75, 295-297). The inhibition of elongation by the nonasaccharide showed a maximum at around 10(-9)M with higher and lower concentrations being less effective. The heptasaccharide did not significantly inhibit elongation at 10(-7)-10(-10)M and also did not affect the inhibition caused by the nonasaccharide when co-incubated with the latter.
从悬浮培养的玫瑰（Rosa“Paul's Scarlet”）细胞细胞壁中提取的半纤维素用绿色木霉纤维素酶进行消化。通过凝胶渗透色谱法对来源于木葡聚糖的九糖和七糖这两种主要的寡糖产物进行了纯化。发现九糖抑制了 2,4-二氯苯氧乙酸诱导的黄化豌豆（Pisum sativum）茎段的伸长。这证实了早先的报道（York 等人，1984，植物生理学，75, 295-297）。九糖对伸长的抑制作用在约 10(-9)M 时达到最大值，较高和较低浓度的抑制作用较小。七糖在 10(-7)-10(-10)M 时对伸长没有显著抑制作用，当与九糖共同孵育时也不会影响后者引起的抑制作用。
