Bose R, Holford-Strevens V, Sehon A H
J Immunol Methods. 1986 May 22;89(2):249-55. doi: 10.1016/0022-1759(86)90365-0.
Four murine monoclonal IgE antibodies specific for the hapten, 4-hydroxy-3-nitrophenylacetyl (NP), had been previously found to be heteroclitic in nature in that they bound the crossreacting hapten, 4-hydroxy-3-iodo-5-nitrophenylacetyl (NIP), with greater affinity than NP. The influence of antibody affinity on the results of two commonly used assays for IgE, namely the radioallergosorbent test (RAST) and histamine release from rat peritoneal mast cells, was studied using these antibodies. In general, in agreement with previous reports, it was found that affinity influences both RAST and histamine release; however, the affinity constants deduced from equilibrium dialysis measurements for the reactions with monovalent haptens were not directly related to the activities of the antibodies as reflected in assays using multivalent hapten protein conjugates.
先前已发现四种对半抗原4-羟基-3-硝基苯乙酰（NP）具有特异性的鼠单克隆IgE抗体本质上是异向性的，因为它们与交叉反应性半抗原4-羟基-3-碘-5-硝基苯乙酰（NIP）结合的亲和力高于NP。使用这些抗体研究了抗体亲和力对两种常用的IgE检测方法结果的影响，即放射变应原吸附试验（RAST）和大鼠腹膜肥大细胞组胺释放试验。总体而言，与先前的报道一致，发现亲和力会影响RAST和组胺释放；然而，通过平衡透析测量得出的与单价半抗原反应的亲和力常数与使用多价半抗原蛋白偶联物的检测中所反映的抗体活性没有直接关系。
