Ren Jianhua, Yang Baichong, Lv Ying, Guo Shuntang
College of Food Science and Nutritional Engineering, China Agricultural University , Haidian District, Beijing , China and.
Int J Food Sci Nutr. 2014 May;65(3):345-50. doi: 10.3109/09637486.2013.854748. Epub 2013 Nov 13.
Peptides derived from alcalase digestion of soybean β-conglycinin, containing 8.52% carbohydrate, exhibits an inhibition effect on pathogen adhesion or translocation to intestinal cells in vitro. In this study, the protective and reparative effects of β-conglycinin peptides on intestinal mucosa injury in vivo were studied using mice with dextran sulfate sodium (DSS)-induced intestinal mucosa injury. The results showed that β-conglycinin peptides contained approximately 21.77% glutamic acid (Glu), and significantly reduced the histological injury in mice both in the protective and reparative experiments. The myeloperoxidase activity of mice treated with β-conglycinin peptides decreased compared with those treated DSS in the positive control group. Immunohistochemical analysis also showed that β-conglycinin peptides inhibited the expression of inflammatory factor NF-κB/p65. These results suggested that peptides derived from soybean β-conglycinin exhibited protective and reparative effects on mice intestinal mucosa injury.
由大豆β-伴大豆球蛋白经碱性蛋白酶消化得到的、含有8.52%碳水化合物的肽，在体外对病原体黏附或向肠细胞的转运具有抑制作用。在本研究中，使用葡聚糖硫酸钠（DSS）诱导肠黏膜损伤的小鼠，研究了β-伴大豆球蛋白肽对体内肠黏膜损伤的保护和修复作用。结果表明，β-伴大豆球蛋白肽含有约21.77%的谷氨酸（Glu），并且在保护和修复实验中均显著减轻了小鼠的组织学损伤。与阳性对照组中用DSS处理的小鼠相比，用β-伴大豆球蛋白肽处理的小鼠的髓过氧化物酶活性降低。免疫组织化学分析还表明，β-伴大豆球蛋白肽抑制炎症因子NF-κB/p65的表达。这些结果表明，大豆β-伴大豆球蛋白衍生的肽对小鼠肠黏膜损伤具有保护和修复作用。
