Laboratório de Tripanosomatídeos, Disciplina de Imunologia, Instituto de Ciências Biomédicas, Universidade Federal de Uberlândia, Av. Amazonas - Bloco 6T sala 07, Campus Umuarama, 38400-902 Uberlândia, Minas Gerais, Brasil.
Glycobiology. 2014 Feb;24(2):179-84. doi: 10.1093/glycob/cwt097. Epub 2013 Nov 12.
The invasion of host cells by the intracellular protozoan Trypanosoma cruzi requires interactions with host cell molecules, and the replication of the parasite requires escape from a parasitophorous vacuole into the host cell cytosol. Galectin-3, a member of β-galactosidase-binding lectin family, has numerous extracellular and intracellular functions. In this study, we investigated the role of galectin-3 during the invasion and intracellular trafficking of T. cruzi extracellular amastigotes (EAs). Endogenous galectin-3 from mouse peritoneal macrophages accumulated around the pathogen during cell invasion by EAs. In addition, galectin-3 accumulated around parasites after their escape from the parasitophorous vacuole. Thus, galectin-3 behaved as a novel marker of phagolysosome lysis during the infection of host cells by T. cruzi.
细胞内原生动物克氏锥虫（Trypanosoma cruzi）入侵宿主细胞需要与宿主细胞分子相互作用，而寄生虫的复制则需要从吞噬小泡逃逸到宿主细胞质中。半乳糖凝集素-3 是β-半乳糖苷酶结合凝集素家族的成员，具有多种细胞外和细胞内功能。在这项研究中，我们研究了半乳糖凝集素-3 在克氏锥虫细胞外无鞭毛体（extracellular amastigotes，EAs）入侵和细胞内运输过程中的作用。在 EAs 感染过程中，来自小鼠腹腔巨噬细胞的内源性半乳糖凝集素-3 在病原体周围聚集。此外，在寄生虫从吞噬小泡逃逸后，半乳糖凝集素-3 也在寄生虫周围聚集。因此，半乳糖凝集素-3 在克氏锥虫感染宿主细胞时表现为一种新的溶酶体裂解标志物。
