Faull R L, Villiger J W
Neuroscience. 1986 Mar;17(3):791-802. doi: 10.1016/0306-4522(86)90045-x.
The anatomical distribution and pharmacological characteristics of benzodiazepine receptors in the human spinal cord were examined in four cases aged 20-41 years using in vitro autoradiography and biochemical assays of [3H]flunitrazepam binding. In all cases, the autoradiograms demonstrated that benzodiazepine receptors were distributed in a consistently similar fashion in the gray matter of the cervical, thoracic, lumbar and sacral regions of the human spinal cord. At all levels, the highest densities of benzodiazepine receptors were found to be localized within lamina II of the dorsal horn as defined on cytoarchitectonic, myeloarchitectonic and substance P immunocytochemical criteria. Within this lamina the receptors were concentrated mainly in its deeper, inner portion which lies immediately adjacent to lamina III, with some overlap dorsally into the outer segment of lamina II and ventrally into the adjacent region of lamina III. The lowest density of receptors was found in regions of laminae I, IV, VII and X; in particular, in lamina VII the lowest concentration of receptors was found in the dorsal nucleus of Clarke and the sacral parasympathetic nucleus. The remaining laminae of the spinal gray (laminae, V, VI, VIII and IX) showed a moderate density of receptors. Biochemical assays of membranes prepared from the lumbosacral cord indicated that these [3H]flunitrazepam binding sites have high affinity and have the pharmacological characteristics of the "central" Type II benzodiazepine receptor. These results show a high concentration of Type II benzodiazepine receptors in the substantia gelatinosa of the human spinal cord and suggest a possible role for these receptors in spinal sensory functions.
利用体外放射自显影技术以及[³H]氟硝西泮结合的生化检测方法，对4例年龄在20至41岁之间的人体脊髓中苯二氮䓬受体的解剖分布和药理学特性进行了研究。在所有病例中，放射自显影片显示，苯二氮䓬受体在人类脊髓颈段、胸段、腰段和骶段灰质中的分布方式始终相似。在所有层面上，根据细胞构筑学、髓鞘构筑学和P物质免疫细胞化学标准确定，苯二氮䓬受体的最高密度位于背角的II层内。在该层内，受体主要集中在其紧邻III层的较深内部，在背侧部分与II层外侧段有部分重叠，在腹侧部分与III层相邻区域有部分重叠。受体密度最低的区域位于I层、IV层、VII层和X层；特别是在VII层，克拉克背核和骶副交感核中的受体浓度最低。脊髓灰质的其余层（V层、VI层、VIII层和IX层）显示出中等密度的受体。对腰骶段脊髓制备的膜进行生化检测表明，这些[³H]氟硝西泮结合位点具有高亲和力，并具有“中枢”II型苯二氮䓬受体的药理学特性。这些结果表明，人类脊髓胶状质中II型苯二氮䓬受体浓度很高，并提示这些受体在脊髓感觉功能中可能发挥作用。
