Department of Agronomy, Kansas State University, 66506, Manhatten, KS, USA.
Theor Appl Genet. 1989 Dec;78(6):783-7. doi: 10.1007/BF00266658.
The ability of immature embryos of wheat (Triticum aestivum L.) to respond to tissue culture has been shown to involve the group 2 chromosomes. The available group 2 ditelosomic and nullisomic-tetrasomic lines of 'Chinese Spring' wheat were used to determine the chromosome arm location and chromosome dosage effect associated with the expression of tissue culture response (TCR). Significant differences were found between the aneuploid lines and the euploid control for the expression of both regenerable callus formation and callus growth rate. A model is proposed suggesting that a major TCR gene is located on 2DL and that 2AL and 2BS possess minor TCR genes. Furthermore, a major regulatory gene controlling the expression of TCR genes may be located on chromosome 2BL.
小麦（Triticum aestivum L.）未成熟胚胎对组织培养的反应能力已被证明与 2 组染色体有关。利用“中国春”小麦的现有 2 组二体和单体-四体缺失系，确定了与组织培养反应（TCR）表达相关的染色体臂位置和染色体剂量效应。在可再生愈伤组织形成和愈伤组织生长速率的表达方面，非整倍体系与整倍体对照之间存在显著差异。提出了一个模型，表明主要的 TCR 基因位于 2DL 上，而 2AL 和 2BS 具有较小的 TCR 基因。此外，控制 TCR 基因表达的主要调控基因可能位于 2BL 染色体上。
