Kato T, Ishihara H, Shimizu A
Neurosci Res. 1986 Feb;3(3):247-52. doi: 10.1016/0168-0102(86)90007-6.
The axonal transport of substance P-hydrolyzing peptidase was studied both 2 and 10 days after the ligation of rat sciatic nerves. A peptidase(s) hydrolyzing substance P at the bonds of Phe7-Phe8 and Phe8-Gly9 was found to have accumulated to about 2 times the normal amount in the proximal segment 10 days after ligation. This enzyme activity was inhibited by ethylenediamine tetraacetate or dithiothreitol. These results suggest that this is a metalloendopeptidase which is slowly transported to inactivate neuropeptides in the nerve terminals.
在大鼠坐骨神经结扎后的第2天和第10天，对P物质水解肽酶的轴突运输进行了研究。发现在结扎后10天，一种在Phe7 - Phe8和Phe8 - Gly9键处水解P物质的肽酶在近端段积累至正常量的约2倍。该酶活性受到乙二胺四乙酸或二硫苏糖醇的抑制。这些结果表明这是一种金属内肽酶，它被缓慢运输以在神经末梢使神经肽失活。
