甘蓝型油菜和芥菜型油菜细胞质雄性不育系的育性恢复。
Male sterility caused by cytoplasm of Brassica oxyrrhina in B. campestris and B. juncea.
机构信息
Biotechnology Centre, Indian Agricultural Research Institute, 110012, New Delhi, India.
出版信息
Theor Appl Genet. 1990 Feb;79(2):285-7. doi: 10.1007/BF00225965.
Synthetic alloploid Brassica oxyrrhina (2n = 18, OO) x B. campestris (2n = 20, AA) was repeatedly backcrossed with B. campestris to place B. campestris nucleus in the cytoplasm of B. oxyrrhina. Alloplasmic plants, obtained in BC5 generation, were stably male sterile but mildly chlorotic during initial development. Synthetic alloploid B. oxyrrhina-campestris was also hybridized with B. juncea to transfer B. oxyrrhina cytoplasm. Segregation for green and chlorotic plants was observed in BC1 and BC2 generations. By selection, however, normal green male sterile B. juncea was obtained in BC3. Pollen abortion in both B. campestris and B. juncea is post-meiotic.
合成异源四倍体 Brassica oxyrrhina（2n = 18，OO）x B. campestris（2n = 20，AA）与 B. campestris 反复回交，将 B. campestris 核置于 B. oxyrrhina 的细胞质中。在 BC5 代获得的异质体植物在初始发育期间稳定雄性不育但轻度黄化。合成异源四倍体 B. oxyrrhina-campestris 也与 B. juncea 杂交，以转移 B. oxyrrhina 细胞质。在 BC1 和 BC2 代观察到绿色和黄化植物的分离。然而，通过选择，在 BC3 代获得了正常的绿色雄性不育 B. juncea。B. campestris 和 B. juncea 中的花粉败育都是减数分裂后发生的。
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