Central Science Laboratory (MAFF), London Road, SL3 7HJ, Slough, Berks, UK.
J Chem Ecol. 1996 Sep;22(9):1639-54. doi: 10.1007/BF02272404.
Coupled GC-EAG techniques have been applied to the study of volatiles from the grain weevil,Sitophilus granarius. for the first time. The size of EAG response was independent of the sex of the responding insect but was consistently larger to extracts of males than those of females. This difference was reflected in a behavioral preference for the male extracts by mated adults of both sexes tested together and virgin adults of both sexes tested separately. The GC-EAG results provide evidence for two materials that are released specifically by the males. Using circular dichroism. one has been found to be identical stereochemically with the (2S,3R)-sitophilate reported by others as the aggregation pheromone in a different strain. This enhances the prospects for the development of a single pheromone lure that would be generally applicable whatever the origin of the strain. The small amount of sitophilate found in the males suggests that it is not stored in large amounts. The other material, present in such a small amount that it has yet to be fully characterized, elicits a higher antennal activity than sitophilate and may have a significant role to play in enhancing the trap catch of this economically important pest.
首次应用耦合 GC-EAG 技术研究了玉米象雄虫的挥发性物质。EAG 反应的大小与昆虫的性别无关，但对雄虫提取物的反应始终大于雌虫提取物。这种差异反映在雌雄成虫共同测试时对雄虫提取物的行为偏好，以及雌雄成虫单独测试时对雄虫提取物的行为偏好。GC-EAG 结果为两种由雄性特异性释放的物质提供了证据。使用圆二色性，一种物质被发现与其他人报道的（2S，3R）-sitophilate 在化学结构上完全相同，后者被报道为另一个不同品系的聚集信息素。这增加了开发单一信息素诱饵的前景，无论品系来源如何，该诱饵都将普遍适用。在雄虫中发现的少量 sitophilate 表明它不是大量储存的。另一种物质，其含量如此之少，以至于尚未完全表征，它引起的触角活性比 sitophilate 高，并且可能在增强这种经济上重要的害虫的诱捕方面发挥重要作用。
