Houben J J, Milaire J
Acta Histochem Suppl. 1986;32:121-6.
Diaminobenzidine (DAB) histochemistry was used to determine enzymatic or non enzymatic peroxidase activity in the rat limb bud on days 12 and 13 of gestation. The optimal technical conditions were determined. Light microscopy revealed the presence of positive intracellular granules in the apical ectodermal ridge (A.E.R.) and in the condensed mesoderm. Their distribution in the A.E.R. shows a caudo-cephalic gradient. Electron microscopy, red oil red, and supravital Nile blue staining suggest the lipidic nature of these granules. The hypothesis of an endogenous pseudo- or peroxidase activity are discussed.
使用二氨基联苯胺（DAB）组织化学法来测定妊娠第12天和第13天大鼠肢芽中的酶促或非酶促过氧化物酶活性。确定了最佳技术条件。光学显微镜显示在顶端外胚层嵴（A.E.R.）和致密中胚层中存在阳性细胞内颗粒。它们在A.E.R.中的分布呈现头尾梯度。电子显微镜、红油红和活体尼罗蓝染色表明这些颗粒具有脂质性质。讨论了内源性假过氧化物酶或过氧化物酶活性的假说。
